Just update to Illustrator 25 and when I try to click on guides they disappear. They also do it when I move and release them. Disabling Real-time drawing or using CPU view does not fix the issue. Very annoying

Me too! Frustrated beyond belief at the time lost over this bug. When costs increase so does the number of bugs. Maybe Adobe needs to reduce the price to reduce the bugs?
I agree, blend Illustrator CC with Affinity today and you'd have the Golden Child of vector applications.
Please post a fix to this issue, ASAP!

Every time I select a guide it disappears, this is totally unacceptable...

Command + E fixed it for me...

Needs fixing ASAP, I'm losing precious design time working around this bug. Begrudged paying a monthly subscription for this not being fixed.

I am experiencing this issue as well. It's suppper annoying. Please help adobe

Yep, known problem.
Please also vote for the more general request about missing items in GPU:
https://illustrator.uservoice.com/forums/601447-illustrator-bugs/suggestions/39740689-realtime-preview-makes-rulers-and-objects-disappea

man adobe sucks.

While in GPU Preview every guideline is disappearing when I grab and drag it. As soon as I let it loose it appears. It's very hard to position a guideline accurately if you can't see it.
In CPU Preview it works, but the overall performance is worse.

Illustrator 25.0
macOS 10.15.7
MacBook Pro 2017 (Radeon Pro 560 4 GB, 16 GB 2133 MHz LPDDR3, 3.1 GHz Quad-Core Intel Core i7)

Have the same annoying problem, they haven't fix it!

Please, fix this big problem. It's important to see the guide while moving it.

Please fix this!

This is really annoying why it isn't implemented yet. Being able to see where guides are going helps out a ton.

Bumping this again in the hopes it gets fixed with the MAX release...

This is an infuriating bug! Thought it was me until I read this page. Jumping in and out of Illustrator and Affinity Designer but that is another story. Mix them together and there is a perfect piece of software.

When the option "Real-time drawing and editing" is activated, the guides disapear when we move them

Apres avoir activé l'option "dessin et travail en temps réel", lorsque l'on déplace un repère celui-ci devient invisible

Extremely Annoying, drives me nuts daily! Used to do this a while back. It then stopped for me. Now since new update its happening none stop again. Very very very frustrating !!!!!!

I can't understand that a bug that was already reported on 09.03.2020 has not been fixed yet.

When moving my guides they become invisible which is bad.