Transparent items appearing as opaque
Shape ontop is 10% opacity but appearing as 100%, but off the artboard is back to 10%
This is strange indeed, Meghan. Disabling GPU should have helped... it's either the file or the specific combination of appearances then... can you reproduce this bug in a new file, with the same exact fills and transparencies? Perhaps you will be able to share it instead of the original then.
Meghan Laidlaw commented
Unfortunately I am not allowed to share the file. Disabling GPU does seem to help the file load as it also glitched a few elements such as text (glitching but showing the text in the previous position when moved to a new position). Even though the GPU was disabled it still doesn't show the transparency...
Also — does disabling GPU helps?
Meghan, can you please provide your exact specs and share the relevant part of you design here (if you are allowed and willing to post it publicly), or via email@example.com (please then provide the link to this issue, https://illustrator.uservoice.com/forums/601447/suggestions/45197365), for the team to study the issue.