There is no GPU Preview option in Illustrator 25.0.1. The closest option I could find in Preferences is the Performance option (see screenshot). The settings in the screenshot are default. What should they be changed to in order to render my guides when dragging or rotating them?

@Katie it seems that is something with the gpu preview. So in the meanwhile change the gpu preview to cpu: ctrl+E / cmd+E

I am having the same issue when I select two guides and try to move or duplicate them by holding alt and dragging on PC (Windows 10), the guides disappear while they are in motion and reappear when they are motionless. It is impossible to use guides effectively with this buggy issue. Please fix ASAP!!

Has anyone found the solution to the guides issue? The guides are there and visible when you create them, but as soon as you try to move them they disappear???!!? This totally defeats the purpose of guides in general...so ******. This needs to be fixed ASAP!!! I wish I never updated illustrator and Photoshop. This is making my life and I'm sure many others much more difficult.

Same problem and, as a teacher, I find the same bug on several different machines/os/gpu. Please fix it asap!

No, it's not. By default "control/command + E" toggles between GPU/CPU rendering modes. On Retina screens with scale CPU mode is incredibly laggy

Guys, Control/Command + E is the solution to this problem.

It's a huge issue! What sense does it make to not be able to see guides and bounding boxes when moving objects?

Same issue! Guides disappear when moving - defeats the purpose!

Bumping this because it's still an issue for many people

Just update to Illustrator 25 and when I try to click on guides they disappear. They also do it when I move and release them. Disabling Real-time drawing or using CPU view does not fix the issue. Very annoying

Me too! Frustrated beyond belief at the time lost over this bug. When costs increase so does the number of bugs. Maybe Adobe needs to reduce the price to reduce the bugs?
I agree, blend Illustrator CC with Affinity today and you'd have the Golden Child of vector applications.
Please post a fix to this issue, ASAP!

Every time I select a guide it disappears, this is totally unacceptable...

Command + E fixed it for me...

Needs fixing ASAP, I'm losing precious design time working around this bug. Begrudged paying a monthly subscription for this not being fixed.

I am experiencing this issue as well. It's suppper annoying. Please help adobe

Yep, known problem.
Please also vote for the more general request about missing items in GPU:
https://illustrator.uservoice.com/forums/601447-illustrator-bugs/suggestions/39740689-realtime-preview-makes-rulers-and-objects-disappea

man adobe sucks.

While in GPU Preview every guideline is disappearing when I grab and drag it. As soon as I let it loose it appears. It's very hard to position a guideline accurately if you can't see it.
In CPU Preview it works, but the overall performance is worse.

Illustrator 25.0
macOS 10.15.7
MacBook Pro 2017 (Radeon Pro 560 4 GB, 16 GB 2133 MHz LPDDR3, 3.1 GHz Quad-Core Intel Core i7)

Have the same annoying problem, they haven't fix it!